Intended for structured formulation workflows, the Hplc Column Nucleodur® C 18 Ec, 5 μm, 300 Å, 150 mm, 4.6 mm is a versatile analytical separation column suitable for separating biomolecular macromolecules such as proteins and peptides. This fully porous particle (FPP) column features a medium density monomeric octadecyl (C 18, ODS) modification, offering a specific surface area of 340 m²/g and a pore size of 300 Å. With a pressure stability of 450 bar and temperature stability of 60 °C, this column is well-suited for high-performance liquid chromatography (HPLC) applications. The column comes packaged with an instruction leaflet and certificate of analysis, making it easy

• Category: Separation columns for HPLC
• USP listing: L1
• Surface chemistry: Medium density monomeric octadecyl (C 18 , ODS) modification
• Particle type: Fully porous particles (FPP)
• pH stability: 1,0-9,0
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Similar phases: NUCLEOSIL ® C 18 Spherisorb ® ODS II Symmetry ® C 18 Hypersil™ ODS Inertsil ® ODS II Kromasil ® C 18 LiChrospher ® RP 18
• Specific surface according to BET: 340 m²/g
• Endcapped: yes
• Eluent in column: Acetonitrile - Water
• Column type: HPLC column
• Column volume: 2.5 ml
• Particle size: 5 µm
• Column shape: Analytical separation column
• Pressure stability: 450 bar
• Separation principle: Reversed phase (RP)
• Temperature stability: 60 ° C
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• Phase: C 18 ec
• Pore volume: 0.9 ml/g
• L x Ø internal (column): 150 x 4.6 mm
• Pore size: 300 Å