Recommended for applications requiring controlled solvent behavior, such as amino acids, highly polar analytes, hydrophilic compounds, nucleosides, oligonucleotides, organic polar acids and bases, peptides, polar natural substances, water-soluble vitamins, and suitable for LC-MS, this HPLC column NUCLEOSHELL ® HILIC features a 100mm x 4.6mm dimension, core-shell, superficially porous particles (SPP), and a particle size of 2.7μm. This column operates under hydrophilic interaction chromatography (HILIC) principles, utilizing ammonium – sulfonic acid (zwitterionic) modification for its surface chemistry. It offers a specific surface area of 130 m²/g, pH stability ranging from 2.0 to

• Category: Separation columns for HPLC
• Column shape: Analytical separation column
• Particle type: Core-shell, superficially porous particles (SPP)
• Pore volume: 0.9 ml/g
• Column type: HPLC column
• L x Ø internal (column): 100 x 4.6 mm
• pH stability: 2,0-8,5
• Endcapped: no
• Particle size: 2.7 µm
• Recommended application(s): Amino acids Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural substances Water-soluble vitamins Suitable for LC-MS
• Column volume: 1.65 ml
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Pressure stability: 600 bar
• Phase: HILIC
• Pore size: 90 Å
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• USP listing: Not available or proprietary
• Temperature stability: 60 ° C
• Specific surface according to BET: 130 m²/g
• Application Database (Macherey-Nagel): HPLC
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Eluent in column: Acetonitrile - Water