Designed with efficiency in synthetic processes in mind, this HPLC column Nucleoshell® Hilic, featuring a length of 100mm and a particle size of 3mm, is ideal for separating highly polar analytes such as amino acids, peptides, and water-soluble vitamins. Its unique zwitterionic surface chemistry, combined with hydrophilic interactions and electrostatic mechanisms, makes it particularly suitable for hydrophilic compounds like nucleosides and oligonucleotides. This column's core-shell, superficially porous particles (SPP), ensure a high specific surface area of 130m²/g, providing excellent separation performance even at pressures up to 600bar and temperatures as high as 60°C. With its recommended applications spanning LC-MS and various polar natural substances, this column is

• Category: Separation columns for HPLC
• Pore size: 90 Å
• Particle type: Core-shell, superficially porous particles (SPP)
• Eluent in column: Acetonitrile - Water
• L x Ø internal (column): 100 x 3 mm
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Endcapped: no
• Pore volume: 0.9 ml/g
• Column volume: 0.7 ml
• Pressure stability: 600 bar
• Temperature stability: 60 ° C
• Column type: HPLC column
• Particle size: 2.7 µm
• pH stability: 2,0-8,5
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Specific surface according to BET: 130 m²/g
• Column shape: Analytical separation column
• USP listing: Not available or proprietary
• Phase: HILIC
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Recommended application(s): Amino acids Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural substances Water-soluble vitamins Suitable for LC-MS
• Application Database (Macherey-Nagel): HPLC