Engineered to maintain reliability in process-driven workflows, the HPLC Column Nucleoshell® Hilic, 50mm, 3mm offers a robust solution for separating highly polar analytes such as amino acids, nucleosides, peptides, and water-soluble vitamins. This analytical separation column features a unique zwitterionic surface chemistry, enabling it to operate effectively under a wide pH range of 2.0-8.5 and withstand pressures up to 600 bar. With a specific surface area of 130 m²/g and a particle size of 2.7 µm, this core-shell, superficially porous particle (SPP) column ensures efficient separation while maintaining high resolution. Ideal for use in liquid chromatography (LC, HPLC), this HILIC column is designed to deliver accurate

• Category: Separation columns for HPLC
• Column shape: Analytical separation column
• Temperature stability: 60 ° C
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Pore size: 90 Å
• Particle type: Core-shell, superficially porous particles (SPP)
• Specific surface according to BET: 130 m²/g
• Pore volume: 0.9 ml/g
• Pressure stability: 600 bar
• Eluent in column: Acetonitrile - Water
• Recommended application(s): Amino acids Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural substances Water-soluble vitamins Suitable for LC-MS
• Phase: HILIC
• Endcapped: no
• Particle size: 2.7 µm
• USP listing: Not available or proprietary
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• L x Ø internal (column): 50 x 3 mm
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• pH stability: 2,0-8,5
• Column volume: 0.35 ml
• Column type: HPLC column