Designed with efficiency in synthetic processes in mind, the HPLC Column Nucleodur® Hilic, 5 μm, 150 mm, 4 mm offers a solution for separating highly polar analytes such as amino acids, peptides, and water-soluble vitamins. This analytical separation column features a hydrophilic interaction chromatography (HILC) phase based on high purity NUCLEODUR® silica gel, providing an alternative strategy for modern analytics, particularly when reversed phase HPLC encounters limitations with polar compounds. With a pH stability range of 2.0-8.5 and temperature stability up to 60°C, this column ensures reliable performance across various experimental conditions. Its particle size of 5 μm, combined with a specific surface area of 340 m²/g, allows for optimal flow rates while

• Category: Separation columns for HPLC
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• pH stability: 2,0-8,5
• Pore size: 110 Å
• Particle type: Fully porous particles (FPP)
• Eluent in column: Acetonitrile - Water
• Phase: HILIC
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Particle size: 5 µm
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Endcapped: no
• Column type: HPLC column
• Column shape: Analytical separation column
• Column volume: 1.9 ml
• Pressure stability: 600 bar
• Specific surface according to BET: 340 m²/g
• L x Ø internal (column): 150 x 4 mm
• Pore volume: 0.9 ml/g
• Temperature stability: 60 ° C
• USP listing: Not available or proprietary