Tailored for precision-driven chemical tasks, the Hplc Column Nucleodur® Hilic, 3 μm, 250 mm, 4 mm offers a unique solution for separating highly polar analytes, such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a zwitterionic HILIC phase based on high purity NUCLEODUR® silica gel, designed to operate under reversed phase HPLC conditions while providing superior separation efficiency for polar compounds. With a pressure stability of 600 bar and temperature stability up to 60°C, this column ensures robust performance even under demanding conditions. The column's specific surface area of 340 m²/g and pH stability range of 2.0-8.5 make it suitable for a wide variety

• Category: Separation columns for HPLC
• Specific surface according to BET: 340 m²/g
• Eluent in column: Acetonitrile - Water
• Phase: HILIC
• Pore volume: 0.9 ml/g
• Column shape: Analytical separation column
• pH stability: 2,0-8,5
• L x Ø internal (column): 250 x 4 mm
• Pressure stability: 600 bar
• Temperature stability: 60 ° C
• Endcapped: no
• Application Database (Macherey-Nagel): HPLC
• Particle size: 3 µm
• USP listing: Not available or proprietary
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Particle type: Fully porous particles (FPP)
• Pore size: 110 Å
• Column type: HPLC column
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Column volume: 3.15 ml
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)