Configured for stable performance in synthesis settings, this HPLC column Nucleodur® Hilic features a particle size of 5 μm and a length of 250 mm, with an internal diameter of 2 mm. It boasts a specific surface area of 340 m²/g, as per BET, and a pore size of 110 Å, offering a pore volume of 0.9 ml/g. This column is ideal for separating highly polar analytes, such as amino acids, peptides, and water-soluble vitamins, using the hydrophilic interaction chromatography (HILIC) principle. Its ammonium – sulfonic acid (zwitterionic) modified surface ensures interactions via both ionic and hydrophilic mechanisms, while its temperature stability extends up to 60°C and pressure stability reaches

• Category: Separation columns for HPLC
• Column shape: Analytical separation column
• Particle size: 5 µm
• USP listing: Not available or proprietary
• Pore volume: 0.9 ml/g
• Particle type: Fully porous particles (FPP)
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Pore size: 110 Å
• Pressure stability: 600 bar
• L x Ø internal (column): 250 x 2 mm
• Eluent in column: Acetonitrile - Water
• Phase: HILIC
• Specific surface according to BET: 340 m²/g
• pH stability: 2,0-8,5
• Column type: HPLC column
• Endcapped: no
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Temperature stability: 60 ° C
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Column volume: 0.8 ml