HPLC column NUCLEODUR ® C 4 ec, 250 mm, 4.6 mm

Developed for demanding processing applications, the Hplc Column Nucleodur® C 4 Ec, 250 mm, 4.6 mm offers exceptional performance in separating biomolecules such as proteins and peptides. This analytical separation column features a medium density monomeric butyl (C 4 ) wide-pore modification, providing a lower retention for hydrophobic compounds compared to other common modifications like C 18 or C 8. With a specific surface area of 100 m²/g, this column ensures efficient separation while maintaining pressure stability up to 450 bar and temperature stability up to 60°C. The column's particle size of 5 µm and fully porous particles (FPP) contribute to its excellent reproducibility and robustness, making it an ideal choice for rigorous laboratory environments.

• Category: Separation columns for HPLC
• Separation principle: Reversed phase (RP)
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• USP listing: L26
• pH stability: 1,0-9,0
• Phase: C 4 ec
• Temperature stability: 60 ° C
• Pressure stability: 450 bar
• Pore size: 300 Å
• Eluent in column: Acetonitrile - Water
• Particle size: 5 µm
• Column type: HPLC column
• Surface chemistry: Medium density monomeric butyl (C 4 ) wide-pore modification
• Pore volume: 0.9 ml/g
• L x Ø internal (column): 250 x 4.6 mm
• Particle type: Fully porous particles (FPP)
• Column shape: Analytical separation column
• Endcapped: yes
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Column volume: 4.15 ml
• Specific surface according to BET: 100 m²/g
• Application Database (Macherey-Nagel): HPLC
• Similar phases: Jupiter ® C 4 ACE ® C 4