HPLC column NUCLEODUR ® HILIC, 1.8 µm, 30 mm, 4.6 mm

A reliable solution for controlled synthesis, the Hplc Column Nucleodur ® Hilic, 1.8 μm, 30 Mm, 4.6 Mm offers a versatile approach for the separation of highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This UHPLC column features a hydrophilic interaction chromatography (HILIC) phase, based on high purity NUCLEODUR ® silica gel, which provides an alternative strategy for modern analytics, particularly when reversed phase HPLC encounters limitations with polar compounds. With a particle size of 1.8 μm, this column ensures efficient separations at high pressures up to 900 bar and temperatures up to 60°C. Its pH stability ranges from 2.

• Category: Separation columns for HPLC
• Pore size: 110 Å
• Pore volume: 0.9 ml/g
• Particle type: Fully porous particles (FPP)
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• L x Ø internal (column): 30 x 4.6 mm
• Specific surface according to BET: 340 m²/g
• Endcapped: no
• Column type: UHPLC column
• USP listing: Not available or proprietary
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Eluent in column: Acetonitrile - Water
• Column volume: 0.49 ml
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Temperature stability: 60 ° C
• pH stability: 2,0-8,5
• Particle size: 1.8 µm
• Pressure stability: 900 bar
• Phase: HILIC
• Column shape: Analytical separation column