HPLC column NUCLEODUR ® HILIC, 3 µm, 250 mm, 4.6 mm

Produced to ensure dependable processing results, the Hplc Column Nucleodur ® Hilic, 3 μm, 250 mm, 4.6 mm offers a versatile solution for separating highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a hydrophilic interaction chromatography (HILIC) phase, which is based on high purity NUCLEODUR ® silica gel, and operates under reversed phase conditions. With a pH stability range of 2.0-8.5, temperature stability up to 60°C, and pressure stability of 600 bar, this column is well suited for a wide variety of applications within the field of liquid chromatography. Additionally, it boasts a specific surface area of 34

• Category: Separation columns for HPLC
• Column shape: Analytical separation column
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Particle type: Fully porous particles (FPP)
• Specific surface according to BET: 340 m²/g
• Temperature stability: 60 ° C
• Phase: HILIC
• Endcapped: no
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• USP listing: Not available or proprietary
• Column volume: 4.15 ml
• Pore size: 110 Å
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Eluent in column: Acetonitrile - Water
• Pore volume: 0.9 ml/g
• Pressure stability: 600 bar
• L x Ø internal (column): 250 x 4.6 mm
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• pH stability: 2,0-8,5
• Column type: HPLC column
• Particle size: 3 µm