HPLC column NUCLEODUR ® NH 2 , 250 mm, 4 mm

Produced to ensure dependable processing results, the Hplc Column Nucleodur ® Nh 2, 250 Mm, 4 Mm offers versatile applications in liquid chromatography. With its aminopropyl modification, this analytical separation column is suitable for both normal phase (NP) and reversed phase (RP) separations. It exhibits a unique retention behavior compared to purely alkyl-functionalized surfaces, making it ideal for analyzing polar compounds such as sugars and DNA bases under RP conditions, as well as hydrocarbons under NP conditions. Its pH stability ranges from 2.0 to 8.0, and it can withstand temperatures up to 60°C. Additionally, this column is capable of anion exchange chromatography of anions and organic acids using common buffers like acetate or phosphate in combination with organic

• Category: Separation columns for HPLC
• Endcapped: no
• Temperature stability: 60 ° C
• Column type: HPLC column
• Particle size: 5 µm
• Specific surface according to BET: 340 m²/g
• Pore volume: 0.9 ml/g
• Separation principle: Multi-mode (NP and RP), Normal phase (NP)
• Eluent in column: n -Heptane
• Application Database (Macherey-Nagel): HPLC
• Column shape: Analytical separation column
• Pressure stability: 600 bar
• Surface chemistry: Aminopropyl (NH 2 ) modification
• Similar phases: NUCLEOSIL ® NH 2 / NH 2 -RP
• USP listing: L8
• Particle type: Fully porous particles (FPP)
• Recommended application(s): Polar compounds under RP conditions (sugars, DNA bases) Hydrocarbons under NP conditions
• Column volume: 3.15 ml
• pH stability: 2,0-8,0
• L x Ø internal (column): 250 x 4 mm
• Interactions / retention mechanism: polar/ionic and hydrophobic
• Phase: NH 2
• Pore size: 110 Å