Adapted for use across diverse chemical handling environments, these Separation columns for HPLC, specifically the Hplc Column Nucleodur ® Hilic, 5 μm, 150 Mm, 4.6 Mm, are designed to cater to the needs of modern analytics. With a pH stability range of 2.0-8.5 and temperature stability up to 60°C, they are suitable for a wide array of applications including amino acids, highly polar analytes, organic polar acids and bases, peptides, polar natural products, water-soluble vitamins, and nucleosides among others. These columns employ the hydrophilic interaction chromatography (HILIC) principle, offering an effective strategy for separating polar analytes in HPLC where reversed phase methods may be limiting. Constructed using fully porous particles (FPP), these

• Category: Separation columns for HPLC
• Particle size: 5 µm
• Temperature stability: 60 ° C
• Column volume: 2.5 ml
• Pressure stability: 600 bar
• USP listing: Not available or proprietary
• Eluent in column: Acetonitrile - Water
• Particle type: Fully porous particles (FPP)
• Column shape: Analytical separation column
• Specific surface according to BET: 340 m²/g
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Phase: HILIC
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Column type: HPLC column
• Pore size: 110 Å
• L x Ø internal (column): 150 x 4.6 mm
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• pH stability: 2,0-8,5
• Pore volume: 0.9 ml/g
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Endcapped: no