HPLC column NUCLEODUR ® PFP, 5 µm, 150 mm, 4.6 mm

Widely applied in chemical workflows, separation columns for High Performance Liquid Chromatography (HPLC) play a crucial role in separating and identifying complex mixtures. One such column is the HPLC column NUCLEODUR ® PFP, featuring a pentafluorophenyl-propyl modification on high purity NUCLEODUR ® silica gel. This hydrophobic phase offers an alternative selectivity compared to traditional C18 modifications, enabling separation through four distinct retention mechanisms: polar interactions (H bonds), dipole-dipole interactions, π-π interactions, and hydrophobic interactions. Its low bleeding characteristics make it suitable for LC/MS applications. The column comes with a particle size of 5 μm, a specific surface area of 340 m²/g, and a recommended temperature range of up to 60°C

• Category: Separation columns for HPLC
• Pressure stability: 600 bar
• Column volume: 2.5 ml
• USP listing: L43
• L x Ø internal (column): 150 x 4.6 mm
• Temperature stability: 60 ° C
• Surface chemistry: Pentafluorophenylpropyl (PFP) modification
• pH stability: 1,0-9,0
• Separation principle: Reversed phase (RP)
• Phase: PFP
• Endcapped: yes
• Interactions / retention mechanism: polar (H-bond), dipole-dipole, π-π and hydrophobic
• Eluent in column: Acetonitrile - Water
• Specific surface according to BET: 340 m²/g
• Pore size: 110 Å
• Recommended application(s): Antibiotics Aromatic and unsaturated compounds Basic compounds Suitable for LC-MS Halogenated compounds Isomers Pharmaceutical products Phenols Especially for polar compounds
• Particle size: 5 µm
• Particle type: Fully porous particles (FPP)
• Column type: HPLC column
• Pore volume: 0.9 ml/g
• Column shape: Analytical separation column