HPLC column NUCLEODUR ® NH 2 -RP, 3 µm, 250 mm, 4.6 mm

Configured for stable performance in synthesis settings, this HPLC column features a particle size of 3 μm and a length of 250 mm, with an inner diameter of 4.6 mm. The column's NH 2 -RP phase, based on high-purity NUCLEODUR® silica gel, offers a unique retention behavior compared to alkyl-functionalized surfaces. It supports both reversed-phase (RP) and normal-phase (NP) separations, making it suitable for a wide range of applications, including polar compounds under RP conditions (such as sugars and DNA bases) and hydrocarbons under NP conditions. With a pH stability range of 2.0-8.0 and a temperature stability of up to 60°C, this column ensures reliable results in various experimental conditions.

• Category: Separation columns for HPLC
• Particle type: Fully porous particles (FPP)
• Column type: HPLC column
• pH stability: 2,0-8,0
• Temperature stability: 60 ° C
• Endcapped: no
• L x Ø internal (column): 250 x 4.6 mm
• Recommended application(s): Polar compounds under RP conditions (sugars, DNA bases) Hydrocarbons under NP conditions
• Application Database (Macherey-Nagel): HPLC
• Pore volume: 0.9 ml/g
• Surface chemistry: Aminopropyl (NH 2 ) modification
• Eluent in column: Acetonitrile - Water
• Pore size: 110 Å
• Pressure stability: 600 bar
• Column volume: 4.15 ml
• Separation principle: Multi-mode (NP and RP), Reversed phase (RP)
• Similar phases: NUCLEOSIL ® NH 2 / NH 2 -RP
• Interactions / retention mechanism: polar/ionic and hydrophobic
• USP listing: L8
• Column shape: Analytical separation column
• Particle size: 3 µm
• Specific surface according to BET: 340 m²/g
• Phase: NH 2 -RP