HPLC column NUCLEODUR ® HILIC, 5 µm, 100 mm, 4.6 mm

Developed for demanding processing applications, the Hplc Column Nucleodur ® Hilic, 5 μm, 100 mm, 4.6 mm offers exceptional performance in separating highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a unique zwitterionic surface chemistry, providing an ideal platform for Hydrophilic Interaction Chromatography (HILIC). With a particle size of 5 μm, it ensures optimal pressure stability up to 600 bar and temperature stability up to 60°C. The column's specific surface area of 340 m²/g, combined with its pH stability range of 2.0-8.5, makes it suitable for a wide variety of applications, including hydrophilic comp

• Category: Separation columns for HPLC
• Phase: HILIC
• Pore size: 110 Å
• Pressure stability: 600 bar
• L x Ø internal (column): 100 x 4.6 mm
• Column shape: Analytical separation column
• Particle type: Fully porous particles (FPP)
• Application Database (Macherey-Nagel): HPLC
• Endcapped: no
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Column type: HPLC column
• Temperature stability: 60 ° C
• Pore volume: 0.9 ml/g
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Eluent in column: Acetonitrile - Water
• USP listing: Not available or proprietary
• Particle size: 5 µm
• Column volume: 1.65 ml
• pH stability: 2,0-8,5
• Specific surface according to BET: 340 m²/g
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification