Manufactured to meet research and production requirements, this Separation column for HPLC, specifically the HPLC column NUCLEODUR ® NH 2 -RP, is designed for versatile applications. With a particle size of 5 μm and a length of 250 mm, it offers a column volume of 4.15 ml. The column is endcapped and features fully porous particles (FPP). It boasts a specific surface area of 340 m²/g, ensuring efficient separations. The column's surface chemistry is aminopropyl (NH 2 ), making it suitable for both reversed phase (RP) and normal phase (NP) separations. The recommended applications include polar compounds under RP conditions (such as sugars and DNA bases) and hydrocarbons under NP conditions. The column is stable up to 60 °C and can

• Category: Separation columns for HPLC
• Specific surface according to BET: 340 m²/g
• Column shape: Analytical separation column
• Pore volume: 0.9 ml/g
• Phase: NH 2 -RP
• L x Ø internal (column): 250 x 4.6 mm
• Column volume: 4.15 ml
• Endcapped: no
• Recommended application(s): Polar compounds under RP conditions (sugars, DNA bases) Hydrocarbons under NP conditions
• USP listing: L8
• Temperature stability: 60 ° C
• Pressure stability: 600 bar
• Pore size: 110 Å
• Application Database (Macherey-Nagel): HPLC SPE
• Similar phases: NUCLEOSIL ® NH 2 / NH 2 -RP
• pH stability: 2,0-8,0
• Eluent in column: Acetonitrile - Water
• Particle size: 5 µm
• Surface chemistry: Aminopropyl (NH 2 ) modification
• Interactions / retention mechanism: polar/ionic and hydrophobic
• Particle type: Fully porous particles (FPP)
• Separation principle: Multi-mode (NP and RP), Reversed phase (RP)
• Column type: HPLC column