Structured to assist in consistent experimental procedures, the Hplc Column Nucleodur ® Hilic, 3 μm, 100 mm, 2 mm is designed for the separation of highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a zwitterionic HILIC phase based on high purity NUCLEODUR ® silica gel, providing an alternative strategy for modern analytics when reversed phase HPLC is often limited. With a pH stability range of 2.0-8.5, temperature stability up to 60°C, and pressure stability of 600 bar, this column is suitable for various laboratory environments. The recommended eluent for this column is acetonitrile-water, making it an ideal choice for hydrophilic compounds

• Category: Separation columns for HPLC
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• pH stability: 2,0-8,5
• Temperature stability: 60 ° C
• Pore volume: 0.9 ml/g
• Column volume: 0.3 ml
• Pore size: 110 Å
• Phase: HILIC
• Particle size: 3 µm
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Pressure stability: 600 bar
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Endcapped: no
• Eluent in column: Acetonitrile - Water
• Column type: HPLC column
• USP listing: Not available or proprietary
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• L x Ø internal (column): 100 x 2 mm
• Particle type: Fully porous particles (FPP)
• Specific surface according to BET: 340 m²/g
• Column shape: Analytical separation column