Intended for structured formulation workflows, the Hplc Column Nucleodur® C 18 Isis, 3 μm, 250 mm, 4 mm offers exceptional steric selectivity as a reversed phase (RP) analytical separation column. With a particle size of 3 μm and a specific surface area of 340 m²/g, this column features fully porous particles (FPP), allowing it to separate positional and structural isomers, planar/non-planar molecules, steroids, fat-soluble vitamins, and (o,p,m)-substituted aromatics. Its nonpolar crosslinked polymeric octadecyl (C 18, ODS) modification ensures high steric selectivity and compatibility with LC-MS applications. The column's pressure stability reaches up to 600

• Category: Separation columns for HPLC
• Temperature stability: 60 ° C
• Interactions / retention mechanism: steric and hydrophobic
• Specific surface according to BET: 340 m²/g
• pH stability: 1,0-10,0
• Pore volume: 0.9 ml/g
• Column type: HPLC column
• Endcapped: yes
• Phase: C 18 Isis
• Surface chemistry: Nonpolar crosslinked polymeric octadecyl (C 18 , ODS) modification, High steric selectivity
• Particle type: Fully porous particles (FPP)
• USP listing: L1
• Recommended application(s): Separation of positional and structural isomers Planar/non-planar molecules Steroids Suitable for LC-MS (o,p,m-)Substituted aromatics Fat-soluble vitamins Exceptional steric selectivity
• Application Database (Macherey-Nagel): HPLC
• Column volume: 3.15 ml
• Column shape: Analytical separation column
• Separation principle: Reversed phase (RP)
• Pressure stability: 600 bar
• Pore size: 110 Å
• Eluent in column: Acetonitrile - Water
• L x Ø internal (column): 250 x 4 mm
• Particle size: 3 µm