HPLC column NUCLEODUR ® C 4 ec, 150 mm, 4.6 mm

Developed for demanding processing applications, the HPLC column Nucleodur® C 4 Ec, 150 mm, 4.6 mm offers exceptional performance in separating biomolecules such as proteins and peptides. This reversed-phase column features a medium density monomeric butyl (C 4 ) wide-pore modification, providing lower retention for hydrophobic compounds compared to C 18 or C 8 modifications. With a specific surface area of 100 m²/g, this analytical separation column boasts a pressure stability of 450 bar and temperature stability up to 60°C. The column's particle size of 5 μm, combined with its fully porous particles (FPP) design, ensures optimal flow rates and reproducibility.

• Category: Separation columns for HPLC
• Pore size: 300 Å
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• Particle size: 5 µm
• Pressure stability: 450 bar
• USP listing: L26
• Column type: HPLC column
• Eluent in column: Acetonitrile - Water
• Surface chemistry: Medium density monomeric butyl (C 4 ) wide-pore modification
• Pore volume: 0.9 ml/g
• Phase: C 4 ec
• Similar phases: Jupiter ® C 4 ACE ® C 4
• pH stability: 1,0-9,0
• Column volume: 2.5 ml
• Temperature stability: 60 ° C
• L x Ø internal (column): 150 x 4.6 mm
• Endcapped: yes
• Separation principle: Reversed phase (RP)
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Column shape: Analytical separation column
• Particle type: Fully porous particles (FPP)
• Specific surface according to BET: 100 m²/g