Produced to ensure dependable processing results, the Hplc Column Nucleodur ® Hilic, 5 μm, 100 mm, 3 mm, offers a reliable solution for separating highly polar analytes, such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a hydrophilic interaction chromatography (HILIC) phase, based on high purity NUCLEODUR ® silica gel, and operates effectively within a pH range of 2.0-8.5 at temperatures up to 60°C and pressures of 600 bar. Its specific surface area, according to BET, is 340 m²/g, ensuring optimal interactions and retention mechanisms through its ionic and hydrophilic characteristics. With a particle size of

• Category: Separation columns for HPLC
• Pore volume: 0.9 ml/g
• Endcapped: no
• Column shape: Analytical separation column
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Phase: HILIC
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Eluent in column: Acetonitrile - Water
• Temperature stability: 60 ° C
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Particle type: Fully porous particles (FPP)
• Pore size: 110 Å
• pH stability: 2,0-8,5
• Particle size: 5 µm
• Column volume: 0.7 ml
• Column type: HPLC column
• Pressure stability: 600 bar
• L x Ø internal (column): 100 x 3 mm
• USP listing: Not available or proprietary
• Specific surface according to BET: 340 m²/g