HPLC column NUCLEODUR ® HILIC, 1.8 µm, 50 mm, 4 mm

Designed for professional use, the Hplc Column Nucleodur ® Hilic, 1.8 μm, 50 Mm, 4 Mm is a UHPLC column suitable for separating highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a hydrophilic interaction chromatography (HILIC) phase based on high purity NUCLEODUR ® silica gel, offering an alternative strategy for the separation of polar analytes in HPLC. With a particle size of 1.8 μm, this column provides excellent pressure stability up to 900 bar and temperature stability up to 60 °C. It is endcapped and has a specific surface area of 340 m²/g according to BET. The

• Category: Separation columns for HPLC
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Column type: UHPLC column
• Phase: HILIC
• Pressure stability: 900 bar
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Specific surface according to BET: 340 m²/g
• L x Ø internal (column): 50 x 4 mm
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Column shape: Analytical separation column
• USP listing: Not available or proprietary
• Pore size: 110 Å
• Temperature stability: 60 ° C
• Eluent in column: Acetonitrile - Water
• Application Database (Macherey-Nagel): HPLC
• Endcapped: no
• Particle size: 1.8 µm
• Particle type: Fully porous particles (FPP)
• Column volume: 0.62 ml
• pH stability: 2,0-8,5
• Pore volume: 0.9 ml/g