Optimized for controlled laboratory processes, the Hplc Column Nucleodur ® Hilic, 3 μm, 150 Mm, 2 Mm offers a unique solution for separating highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a hydrophilic interaction chromatography (HILIC) phase based on high purity NUCLEODUR ® silica gel, providing an alternative strategy for modern analytics, particularly when reversed phase HPLC encounters limitations with polar compounds. With a pH stability range of 2.0-8.5, temperature stability up to 60°C, and pressure stability of 600 bar, this column ensures reliable performance even under challenging conditions. The Zwitterionic HILIC phase's surface

• Category: Separation columns for HPLC
• pH stability: 2,0-8,5
• Pore size: 110 Å
• Particle type: Fully porous particles (FPP)
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Specific surface according to BET: 340 m²/g
• USP listing: Not available or proprietary
• Column shape: Analytical separation column
• L x Ø internal (column): 150 x 2 mm
• Particle size: 3 µm
• Endcapped: no
• Phase: HILIC
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Column volume: 0.45 ml
• Pressure stability: 600 bar
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Temperature stability: 60 ° C
• Column type: HPLC column
• Pore volume: 0.9 ml/g
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Eluent in column: Acetonitrile - Water