Intended for structured formulation workflows, the Hplc Column Nucleodur® C 18 Ec, 5 μm, 300 Å, 250 mm, 4 mm is a valuable addition to any lab specializing in liquid chromatography (LC, HPLC). This analytical separation column features a medium density monomeric octadecyl (C 18, ODS) modification, making it ideal for separating biomolecular macromolecules such as proteins and peptides. With a specific surface area of 340 m²/g, this column offers excellent efficiency and resolution. Its particle size of 5 μm ensures high pressure stability up to 450 bar and temperature stability up to 60°C. The column's Pore size of 300 Å allows for a wide range of applications, including the separation of

• Category: Separation columns for HPLC
• Surface chemistry: Medium density monomeric octadecyl (C 18 , ODS) modification
• Pore volume: 0.9 ml/g
• Separation principle: Reversed phase (RP)
• Pressure stability: 450 bar
• Application Database (Macherey-Nagel): HPLC
• Column volume: 3.15 ml
• Column shape: Analytical separation column
• Pore size: 300 Å
• Endcapped: yes
• Eluent in column: Acetonitrile - Water
• L x Ø internal (column): 250 x 4 mm
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Particle size: 5 µm
• Specific surface according to BET: 340 m²/g
• Phase: C 18 ec
• Similar phases: NUCLEOSIL ® C 18 Spherisorb ® ODS II Symmetry ® C 18 Hypersil™ ODS Inertsil ® ODS II Kromasil ® C 18 LiChrospher ® RP 18
• USP listing: L1
• Temperature stability: 60 ° C
• pH stability: 1,0-9,0
• Column type: HPLC column
• Particle type: Fully porous particles (FPP)
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions