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HPLC column NUCLEODUR ® C 18 Gravity SB, 5 µm, 250 mm, 4.6 mm

Order number: 340Y.1

Part number: AC100008731

£ 759.51*

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£ 911.41*

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Produced for stability across temperature-sensitive applications, this HPLC column belongs to the category of separation columns designed for liquid chromatography. Made of high purity NUCLEODUR® silica gel, it features a C 18 Gravity SB hydrophobic phase that offers distinct polar selectivity for improved retention of early eluting substances. With a pore size of 110 Å and a specific surface area of 340 m²/g, it demonstrates exceptional performance under highly aqueous conditions. Its endcapped nature ensures low bleeding characteristics, making it suitable for LC/MS applications. This column is ideal for overall sophisticated analytical separations, particularly for polar compounds, and exhibits excellent performance under temperatures up to 60°C and pressures up to 600 bar.

  • Category: Separation columns for HPLC
  • Separation principle: Reversed phase (RP)
  • Phase: C 18 Gravity SB
  • Particle type: Fully porous particles (FPP)
  • L x Ø internal (column): 250 x 4.6 mm
  • Pore volume: 0.9 ml/g
  • Pressure stability: 600 bar
  • Temperature stability: 60 ° C
  • Column volume: 4.15 ml
  • Particle size: 5 µm
  • Endcapped: yes
  • Application Database (Macherey-Nagel): HPLC
  • Interactions / retention mechanism: hydrophobic (van der Waals) with additional polar interactions
  • Column type: HPLC column
  • Similar phases: NUCLEOSIL ® C 18 HD Xterra ® RP 18 / MS C 18 Luna ® C 18 (2) Gemini ® Synergi™ Max RP Zorbax ® Extend C 18 Inertsil ® ODS III Purospher ® Star RP-18 Hypersil™ BDS
  • Recommended application(s): Overall sophisticated analytical separations Antibiotics Organic acids Ideal for method development Especially for polar compounds Water soluble vitamins Excellent performance under highly aqueous conditions
  • Eluent in column: Acetonitrile - Water
  • Surface chemistry: Base deactivated (BDS), High density octadecyl (C 18 , ODS) modification
  • Pore size: 110 Å
  • Specific surface according to BET: 340 m²/g
  • USP listing: L1
  • pH stability: 1,0-9,0
  • Column shape: Analytical separation column