Produced for stability across temperature-sensitive applications, the Hplc Column Nucleodur® PFP, 3 μm, 250 mm, 4.6 mm offers versatile separation capabilities within liquid chromatography (LC, HPLC). Based on high-purity NUCLEODUR® silica gel, this hydrophobic phase features a pentafluorophenyl-propyl modification that provides an alternative selectivity compared to traditional C18 modifications. Its unique surface chemistry supports four retention mechanisms – polar interactions (H bonds), dipole-dipole interactions, π-π interactions, and hydrophobic interactions – making it suitable for LC/MS due to its low bleeding characteristics. With a recommended operating range of 1.0-9.0 pH, this analytical separation column exhibits pressure stability up to 600 bar and temperature stability at

• Category: Separation columns for HPLC
• Phase: PFP
• Column type: HPLC column
• Specific surface according to BET: 340 m²/g
• Column volume: 4.15 ml
• pH stability: 1,0-9,0
• USP listing: L43
• Particle type: Fully porous particles (FPP)
• Pore volume: 0.9 ml/g
• Temperature stability: 60 ° C
• Recommended application(s): Antibiotics Aromatic and unsaturated compounds Basic compounds Suitable for LC-MS Halogenated compounds Isomers Pharmaceutical products Phenols Especially for polar compounds
• Pore size: 110 Å
• Eluent in column: Acetonitrile - Water
• Separation principle: Reversed phase (RP)
• Particle size: 3 µm
• L x Ø internal (column): 250 x 4.6 mm
• Pressure stability: 600 bar
• Endcapped: yes
• Column shape: Analytical separation column
• Surface chemistry: Pentafluorophenylpropyl (PFP) modification
• Interactions / retention mechanism: polar (H-bond), dipole-dipole, π-π and hydrophobic