Structured to assist in consistent experimental procedures, this Hplc Column Nucleoshell ® Hilic, measuring 100 mm in length and featuring a 2 mm diameter, is designed for use in liquid chromatography (LC, HPLC). Its core-shell, superficially porous particle (SPP) construction ensures optimal performance, while its ammonium – sulfonic acid (zwitterionic) modification facilitates separations through hydrophilic interaction chromatography (HILC). This column is particularly suitable for the separation of highly polar analytes such as amino acids, nucleosides, oligonucleotides, organic polar acids and bases, peptides, polar natural substances, water-soluble vitamins, and is also compatible with LC-MS applications. With a specific surface area of 130 m²/g, a pore

• Category: Separation columns for HPLC
• Endcapped: no
• Temperature stability: 60 ° C
• Specific surface according to BET: 130 m²/g
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Recommended application(s): Amino acids Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural substances Water-soluble vitamins Suitable for LC-MS
• Pore volume: 0.9 ml/g
• Column type: HPLC column
• L x Ø internal (column): 100 x 2 mm
• pH stability: 2,0-8,5
• Particle size: 2.7 µm
• Eluent in column: Acetonitrile - Water
• Phase: HILIC
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Pore size: 90 Å
• Pressure stability: 600 bar
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Column shape: Analytical separation column
• Particle type: Core-shell, superficially porous particles (SPP)
• USP listing: Not available or proprietary
• Column volume: 0.3 ml