Designed for professional use, the Hplc Column Nucleodur® C 4 Ec, 250 Mm, 3 Mm is an ideal choice for separating biomolecules such as proteins and peptides. This reversed-phase (RP) analytical separation column features a medium density monomeric butyl (C 4 ) wide-pore modification, providing a lower retention for hydrophobic compounds compared to other common modifications like C 18 or C 8. With a particle size of 5 µm, fully porous particles (FPP), and a pressure stability of 450 bar, this column ensures efficient and reliable performance under various conditions. Its specific surface area according to BET is 100 m²/g, offering ample interaction sites for effective separations. The column's recommended eluent is acetonitrile-water, and it

• Category: Separation columns for HPLC
• Separation principle: Reversed phase (RP)
• Column shape: Analytical separation column
• Pore size: 300 Å
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• Pressure stability: 450 bar
• pH stability: 1,0-9,0
• Eluent in column: Acetonitrile - Water
• Column type: HPLC column
• Similar phases: Jupiter ® C 4 ACE ® C 4
• Particle type: Fully porous particles (FPP)
• Endcapped: yes
• Pore volume: 0.9 ml/g
• Column volume: 1.75 ml
• Phase: C 4 ec
• Surface chemistry: Medium density monomeric butyl (C 4 ) wide-pore modification
• Temperature stability: 60 ° C
• L x Ø internal (column): 250 x 3 mm
• Specific surface according to BET: 100 m²/g
• Particle size: 5 µm
• USP listing: L26