Coelenterazine is available in professionally graded form for technical applications. It has a molecular formula of C26H46I2N2 and a molecular weight of 640.5. This compound belongs to the class of organoiodine compounds, which are characterized by the presence of iodine atoms bonded to carbon. Coelenterazine requires careful handling due to its sensitivity to light and air. It finds applications in various biochemical experiments, particularly as a substrate for the enzyme Renilla luciferase, where it plays a key role in monitoring calcium flux and reporter genes. Additionally, it is utilized in bioluminescence resonance energy transfer (BRET) assays, ELISAs, high-throughput screening (HTS), and in the chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.

• CAS Number: 55779-48-1
• Exact Mass: 640.17504
• Molecular Weight: 640.5
• Molecular Formula: C26H46I2N2