X-Gluc is a documented reagent used for microbiological analysis, specifically for the colorimetric detection of glucuronidase activity. Its molecular formula is C20H26BrClN2O7, with a molecular weight of 521.8. This compound belongs to a class of organic compounds containing bromine, chlorine, nitrogen, oxygen, and carbon. It is important to handle this reagent with caution, as indicated by the Warning signal word due to its potential harmfulness if ingested. During hydrolysis, X-Gluc breaks down into glucuronic acid and 5-bromo-4-chloro-indoxyl, which is further oxidized to form a deep-blue indigo dye. This reaction allows for the visual detection of enzymatic activity in various biological samples.

• CAS Number: 114162-64-0
• Molecular Formula: C20H26BrClN2O7
• GHS Signal Word: Warning
• Molecular Weight: 521.8
• Exact Mass: 520.06119
• Hazard Statements:
• H302: Harmful if swallowed [Warning Acute toxicity, oral]