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Lactose Broth for microbiology Figure: (1) Escherichia coli (gas formation (arrow)). (2) Uninoculated broth.
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Sorbitol MacConkey Agar ISO 11133, ISO 16654, for microbiology Figure: A: Escherichia coli ATCC 25922. B: Escherichia coli O157:H7.
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Lauryl Sulphate Broth ISO 4831:2006, ISO 7251, ISO 11133, for microbiology
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Tryptophane Culture Broth DEV, ISO 16654, for microbiology Escherichia transform lactose under acid production via β-galactosidase, and are negative in oxidase test and positive in indole production test. Kovac Reagent (Art. No. 2950.1) is added to 24-48 h old cultures in a concentration of 0.2 to 0.5 ml per 5 ml broth. A colour shift to dark red shows presence of indole.
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Lysine Iron Agar for microbiology Differentiation of Enterobacteriaceae on the basis of Lysine decarboxylation and -deamination, as well as of H 2 S production.
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Endo Agar (Base) for microbiology Figure: Escherichia coli grows red colonies accompanied by red staining of the surrounding medium (sodium sulphite/fuchsin reaction).
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MacConkey Broth acc. to Ph. Eur./USP, for microbiology Composition corresponds to Ph. Eur. and USP
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Mossel EE Broth acc. to Ph. Eur./USP, for microbiology Composition corresponds to Ph. Eur. and USP Figure: (1) Enterobacteria (here: E. coli ), indicated by colour yellow caused by pH-shift. (2) Uninoculated broth.
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Nutritionally reduced medium. Incubation of water samples on R2A Agar often leads to higher colony numbers of micro organisms than found for rich media, particularly when incubation is performed at lower temperatures. The medium was originally developed by Reasoner and Geldreich to determine the total germ number in drinking water (Reasoner and Geldreich, 1985, Appl. Environ. Microbiol. 49:1). R2A-Agar acc. to Ph. Eur., for microbiology Composition corresponds to Ph. Eur.
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