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This is the DNA dye BrightGreen I, a staining reagent for detection of double stranded nucleic acid in agarose and polyacrylamide gels. The dye is added directly to the DNA/loading buffer mixture and then loaded onto the gel. BrightGreen I bounds to the minor groove of DNA and intercalates into the DNA. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide. BrightGreen I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects. Recommended for dsDNA Direct addition to DNA sample For excitation with UV light and blue light Non-toxic alternative for ethidium bromide Sensitivity (0,01 ng/band) BrightGreen DNA dye can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). The gels may be documented by all usual broadband ethidium bromide- or BrightGreen foto filters. Wavelength Excitation maximum (bound to DNA): approx. 254 nm and 495 nm Emission maximum (bound to DNA): 521 nm BrightGreen DNA dye 11x conc., for electrophoresis, ready-to-use
Product Detail
This is the DNA dye BrightGreen I, a staining reagent for detection of double stranded nucleic acid in agarose and polyacrylamide gels. The dye is added directly to the DNA/loading buffer mixture and then loaded onto the gel. BrightGreen I bounds to the minor groove of DNA and intercalates into the DNA. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide. BrightGreen I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects. Recommended for dsDNA Direct addition to DNA sample For excitation with UV light and blue light Non-toxic alternative for ethidium bromide Sensitivity (0,01 ng/band) BrightGreen DNA dye can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). The gels may be documented by all usual broadband ethidium bromide- or BrightGreen foto filters. Wavelength Excitation maximum (bound to DNA): approx. 254 nm and 495 nm Emission maximum (bound to DNA): 521 nm BrightGreen DNA dye 11x conc., for electrophoresis, ready-to-use
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
