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ROTI ® Prep Plasmid MINI-XL for molecular biology New - With optimised protocol for isolation from 0.5-2.0 ml bacterial culture ! MIDI preps in MINI size-fomat. ROTI ® Prep Plasmid MINI-XL is a further development of the classical Plasmid Mini extraction kit, which is based on an alkaline lysis and binding of the plasmid to the spin filter. The binding capacity of the spin filter has been, compared to usual plasmid mini kits, considerably increased (approx. 80 μg plasmid DNA) and allows now a plasmid extraction from 0.5-15 ml bacteria culture. Since filter dimensions correspond to the usual miniprep spin filters, its application differs only slightly from other plasmid mini kits. Most significant difference is the afore done convenient lysis in a 15 ml tube. Preparation by well-known mini spin-column system Fast, easy and efficient Preparation from 0.5-15 ml bacteria culture Preparation within approx. 20 minutes Yield 40-60μg (average per 15 ml bacterial culture). The elution resulting in highly concentrated pure DNA in 100 μl, takes then place in a 1,5 ml tube, like in standard mini kits. Isolated DNA is free of RNA or protein contamination and can be directly applied to all standard down-stream applications like restriction digest, sequencing, in vitro transcription, transfection etc. The kit contains: Resuspension Buffer RB, Lysis Buffer LS, Neutralisation Buffer NB, Washing Buffer PA, Washing Solution PB, Elution Buffer XEB, spin columns, collection tubes, elution tubes, detailed instructions-for-use. Contents of this kit may not be bought separately. Please order ethanol 96-99,8 % (p.a., Art. No. 9065) separately.
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NucleoSpin Gel and PCR Clean-up Mini Kit for gel extraction and PCR clean up NucleoSpin ® Not a medical device / Not an IVD product
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ROTI ® Prep Gel & PCR for molecular biology ROTI ® Prep Gel & PCR is designed to provide the greatest flexibility in the purification of isolated DNA fragments. The kit is used for the purification of DNA from PCR reactions as well as for the purification from gel pieces. The purification from PCR and sequencing reactions is based on a new two-step process in which the DNA is first bound to the column membrane using the Binding Buffer and then eluted in the second step. In the purification from agarose gel pieces, the gel piece is first dissolved and the DNA is then washed in several washing and centrifugation steps before it can be eluted. Great flexibility through dual use Preparation in the well-established mini-column system Fast, easy and reliable Extraction time: approx. 3 min (sequencing and PCR reactions), approx. 20 min (gel extraction) Possible basic material: TAE and TBE agarose gels (up to 300 mg) PCR reactions (up to 50 µl) Sequencing reactions (up to 50 µl) The kit contains: Gel Solubilizer, Binding Enhancer, Binding Buffer, Washing Solution WSL (conc.), Elution Buffer EB, centrifugation columns, collection tubes. Elution tubes, detailed instructions. Ethanol 99.8 % (p.a., Art. no. 9065) is not included in the kit and should be ordered separately if required. Not a medical device / Not an IVD product
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NucleoSpin DNA Stool, Mini kit for DNA from stool NucleoSpin ® Not a medical device / Not an IVD product
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NucleoSpin Plant II, Mini kit for DNA from plants NucleoSpin ® Not a medical device / Not an IVD product
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ROTI ® Prep Soil DNA for molecular biology ROTI ® Prep Soil DNA was developed to isolate microbial DNA from soil samples. Soil samples represent a particularly complex starting material, which is why it is urgently necessary to first mechanically decompose it. In the first step of extraction, the soil samples are homogenised and disrupted in a lysis tube in lysis buffer. After a centrifugation step, the lysate is transferred to the centrifugation column and the DNA is bound to the membrane with the aid of the binding buffer. After several washing and centrifugation steps, the DNA is then eluted from the membrane with the elution buffer. Specially developed for DNA extraction from complex soil samples Preparation in the well-established mini-column system Fast, easy and reliable Extraction time of approx. 45 min Possible basic material: Soil sample (up to 400 mg) The kit contains: ROTI ® SampleLyse Microbes 1 tubes, Lysis Buffer LSS, Precipitation Buffer PB, Binding Buffer BR, Binding Buffer BDS, Washing Solution WSO, Washing Buffer WSH (conc.), Elution Buffer EB, detailed instructions. Ethanol 99.8 % (p.a., Art. no. 9065) and a homogeniser (e.g. Art. no. PA66.1) are not included in the kit and should be ordered separately if required. Not a medical device / Not an IVD product
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ROTI ® Prep Plant RNA for molecular biology ROTI ® Prep Plant RNA is designed to isolate total RNA from various plant starting materials. The isolation is done from homogenised plant material, which is then treated with one of the lysis buffers included in the kit to break down even complex plant components. The lysed sample is then filtered through a centrifugation column, which binds the DNA. The filtrate is then transferred to a second centrifugation column that binds the RNA. After several washing and centrifugation steps, the RNA is eluted from the membrane by the elution buffer and is now ready for use in all subsequent applications. Specially developed for extraction from plant materials Preparation by well-known mini spin-column system Fast, easy and efficient Preparation in approx. 30 min Yield up to 70 µg RNA, depending on source material Use of DNase not necessary Possible basic material: Blossoms Leaves Roots Stems Wooden parts Seeds Grains The kit contains: Lysis Buffer LSR, Lysis Buffer LSK, Washing Buffer WSA (conc.), Washing Solution WSL (conc.) RNase-free Water, centrifugation columns DNA (blue), centrifugation columns RNA (violet), collection tubes, elution tubes. Ethanol 99.8 % (p.a., Art. no. 9065) is not included in the kit and should be ordered separately if required. Not a medical device / Not an IVD product
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NucleoSpin Soil, Mini kit for DNA from soil NucleoSpin ® Not a medical device / Not an IVD product
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NucleoSpin DNA Stool, Mini kit for DNA from stool NucleoSpin ® Not a medical device / Not an IVD product
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NucleoSpin RNA Plant, Mini kit for RNA plant NucleoSpin ® Not a medical device / Not an IVD product
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ROTI ® Prep RNA MINI for molecular biology Roti ® Prep RNA MINI has been developed for the isolation of total RNA from various sources. The isolation is carried out through the proven and reliable spin column technique, which is very easy to handle and needs only few steps. Preparation by well-known mini spin-column system Fast, easy and efficient Use of DNase not necessary Preparation in approx. 15 to 40 minutes Yields up to 100 μg RNA, depending on source material Elution in 30-80 μl One of the critical points in RNA extraction is the presence of endogenous RNases, which can lead to high losses very early in extraction of the (in comparison to DNA) very sensitive RNA. Therefores the lysis buffer has been specially optimised to inactivate RNases, with highest efficiency, already at the early stage of the extraction. Furthermore, in order to get a high-pure total RNA, one particularly for this purpose developed prefilter is used. This filter exclusively serves for binding and targeted removal of DNA impurities, and is discarded after use with the bound DNA. Thus, it is possible to omit application of DNase I. Total RNA is then obtained from the DNA-free eluate through a simple “Binding-Washing-Elution” process. In each preparation, up to 100 μg highly pure total RNA may be isolated. One preparation takes (excluding lysis) approx. 10 mins. Isolated RNA is free of DNA and enzyme inhibitors, and may directly be applied to RT-PCR or other downstream applications. Possible basic material: Tissues (up to 20 mg) Eukaryotic cells (up to 5x10 6 ) Bacteria Gram + and Gram - (up to 1x10 9 ) Biopsies The kit contains: Lysis Buffer LSR, Washing Buffer WSA, Washing Solution WSL, RNase-free Water, spin filters (blue), spin columns (violet), collection tubes, elution tubes, detailed instructions-for-use. Contents of this Kit may not be bought separately. Please order ethanol 99,8 % (p.a., Art. No. 9065) and 70 % (p.a., Art. No. T868) separately. Not a medical device / Not an IVD product
Product Detail
ROTI ® Prep Plant RNA for molecular biology ROTI ® Prep Plant RNA is designed to isolate total RNA from various plant starting materials. The isolation is done from homogenised plant material, which is then treated with one of the lysis buffers included in the kit to break down even complex plant components. The lysed sample is then filtered through a centrifugation column, which binds the DNA. The filtrate is then transferred to a second centrifugation column that binds the RNA. After several washing and centrifugation steps, the RNA is eluted from the membrane by the elution buffer and is now ready for use in all subsequent applications. Specially developed for extraction from plant materials Preparation by well-known mini spin-column system Fast, easy and efficient Preparation in approx. 30 min Yield up to 70 µg RNA, depending on source material Use of DNase not necessary Possible basic material: Blossoms Leaves Roots Stems Wooden parts Seeds Grains The kit contains: Lysis Buffer LSR, Lysis Buffer LSK, Washing Buffer WSA (conc.), Washing Solution WSL (conc.) RNase-free Water, centrifugation columns DNA (blue), centrifugation columns RNA (violet), collection tubes, elution tubes. Ethanol 99.8 % (p.a., Art. no. 9065) is not included in the kit and should be ordered separately if required. Not a medical device / Not an IVD product
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
