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The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA (with glycerol) 6x conc., DNase-free Directions for use In order to cover the main size reange needed in gel electrophoresis, ROTI ® Load DNA (with glycerol) contains bromophenol blue and xylene cyanol (see lane 1 in the product image). Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
Product Detail
The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA (with ficoll) 6x conc., DNase-free Directions for use In order to cover the main size reange needed in gel electrophoresis, ROTI ® Load DNA (with ficoll) contains bromophenol blue and xylene cyanol (see lane 1 in the product image). Ficoll ® 400 produces particularly sharp bands and is often used when smaller amounts of DNA are to be depicted in the gel. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
Product Detail
The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA small (with glycerol) 6x conc., DNase-free Directions for use ROTI ® Load DNA small (with glycerol) s a special gel loading buffer for gel electrophoresis of small fragments in which only xylene cyanol was used as dye. The buffer thus efficiently avoids the covering of important DNA bands in the small fragment area and enables a clean presentation, even in highly concentrated agarose gels (see lane 3 in the product image). Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
Product Detail
Loading bufer with the fluorescent dye SYBR ® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels. Alternative for ethidium bromide, non-toxic, non-mutagen 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band For use with common broad-band ethidium bromide photo filters Excitation possible via UV light (254 nm) and blue light (495 nm) Compatible with all usual down-stream applications. ROTI ® Load DNAstain SYBR ® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR ® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR ® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects. SYBR ® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). When excited, nucleic acid-bound SYBR ® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR ® Green foto filters. The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI ® Load DNAstain SYBR ® Green. Wavelength Excitation maximum (bound to DNA): 254 nm and 495 nm Emission maximum (bound to DNA): 521 nm ROTI ® Load DNAstain 2 Green 6x conc., ready-to-use Directions for use ROTI ® Load DNAstain 2 Green contains tartrazine and xylencyanol to mark the length. Tartrazine runs in the very small fragment range and shows the maximum running distance for very short runs. Recommended if bromophenol blue could mask important DNA bands. Glycerin is the standard reagent for increasing sample density. Recommended for staining of dsDNA- and dsRNA-fragments of 100-2000 bp.
Product Detail
The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA short-run 1x (with glycerol) 1x conc., ready-to-use, DNase-free Directions for use In order to prevent important DNA bands being clouded, ROTI ® Load DNA short-run 1x (with glycerol) contains only bromophenol blue in slightly reduced concentration (see lane 2 in the product image). The buffer may also be diluted to 0,6x if the bromophenol blue in the gel could mask an important DNA band. Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
Product Detail
The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA-tricolor (with glycerol) 6x conc., DNase-free Directions for use ROTI ® Load DNA-tricolor (with glycerol) is a gel loading buffer with orange G, bromophenol blue and xylencyanol as dyes (see lane 4 in the product image). Perfectly suited if a large fragment range is to be separated in the gel. Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
Product Detail
Loading bufer with the fluorescent dye SYBR ® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels. Alternative for ethidium bromide, non-toxic, non-mutagen 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band For use with common broad-band ethidium bromide photo filters Excitation possible via UV light (254 nm) and blue light (495 nm) Compatible with all usual down-stream applications. ROTI ® Load DNAstain SYBR ® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR ® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR ® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects. SYBR ® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). When excited, nucleic acid-bound SYBR ® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR ® Green foto filters. The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI ® Load DNAstain SYBR ® Green. Wavelength Excitation maximum (bound to DNA): 254 nm and 495 nm Emission maximum (bound to DNA): 521 nm ROTI ® Load DNAstain 3 Green 6x conc., ready-to-use Directions for use ROTI ® Load DNAstain 3 Green is a special gel loading buffer with orange G as the only tracking dye optimized for very short runs. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs. Also well suited if bromophenol blue or xylene cyanol could mask important DNA bands. Glycerin is the standard reagent for increasing sample density. Recommended for staining of dsDNA- and dsRNA-fragments of under 500 bp.
Product Detail
The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA orange 1 (with glycerol) 6x conc., DNase-free Directions for use ROTI ® Load DNA-orange 1 (with glycerol) is a special gel loading buffer with orange G as the only dye optimized for very short runs. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs (see lane 6 in the product image). Also well suited if bromophenol blue or xylene cyanol could mask important DNA bands. Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
Product Detail
The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA 1x (with glycerol) 1x conc., ready-to-use, DNase-free Directions for use In order to cover the main size reange needed in gel electrophoresis, ROTI ® Load DNA 1x (with glycerol) contains bromophenol blue and xylene cyanol (see lane 1 in the product image). Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
Product Detail
Loading bufer with the fluorescent dye SYBR ® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels. Alternative for ethidium bromide, non-toxic, non-mutagen 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band For use with common broad-band ethidium bromide photo filters Excitation possible via UV light (254 nm) and blue light (495 nm) Compatible with all usual down-stream applications. ROTI ® Load DNAstain SYBR ® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR ® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR ® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects. SYBR ® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). When excited, nucleic acid-bound SYBR ® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR ® Green foto filters. The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI ® Load DNAstain SYBR ® Green. Wavelength Excitation maximum (bound to DNA): 254 nm and 495 nm Emission maximum (bound to DNA): 521 nm ROTI ® Load DNAstain 3 Green 6x conc., ready-to-use Directions for use ROTI ® Load DNAstain 3 Green is a special gel loading buffer with orange G as the only tracking dye optimized for very short runs. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs. Also well suited if bromophenol blue or xylene cyanol could mask important DNA bands. Glycerin is the standard reagent for increasing sample density. Recommended for staining of dsDNA- and dsRNA-fragments of under 500 bp.
Product Detail
Loading bufer with the fluorescent dye SYBR ® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels. Alternative for ethidium bromide, non-toxic, non-mutagen 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band For use with common broad-band ethidium bromide photo filters Excitation possible via UV light (254 nm) and blue light (495 nm) Compatible with all usual down-stream applications. ROTI ® Load DNAstain SYBR ® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR ® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR ® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects. SYBR ® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE ® PROfessional runVIEW, Art. No. 4849.1). When excited, nucleic acid-bound SYBR ® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR ® Green foto filters. The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI ® Load DNAstain SYBR ® Green. Wavelength Excitation maximum (bound to DNA): 254 nm and 495 nm Emission maximum (bound to DNA): 521 nm ROTI ® Load DNAstain 1 Green 6x conc., ready-to-use Directions for use In order to cover the main size range needed in gel electrophoresis, ROTI ® Load DNAstain 1 Green contains bromophenol blue and xylene cyanol. Glycerin is the standard reagent for increasing sample density. Recommended for staining of dsDNA- and dsRNA-fragments of over 500 bp.
Product Detail
The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly. ROTI ® Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose. Working concentration ROTI ® Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI ® Load DNA. ROTI ® Load DNA gel loading buffer is usually used as 1x concentrated solution. ROTI ® Load DNA orange 2 (with glycerol) 6x conc., DNase-free Directions for use ROTI ® Load DNA-orange 2 (with glycerol) is a gel loading buffer with orange G and xylencyanol as dyes. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs (see lane 5 in the product image). Well suited if a large fragment range is to be separated in the gel or if bromophenol blue could mask important DNA bands. Glycerin is the standard reagent for increasing sample density. Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
Product Detail© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
